Identification of iron-reducing bacteria isolated from soil ferrosphere

Abstract

The aim of the present research was to isolate and identify iron-reducing bacteria from the soil ferrosphere using microbiological, physiological-biochemical and molecular genetic methods, to analyze their ability to produce some corrosive dangerous metabolites. ChNPU ZVB1 strain was isolated and selected as one with iron-reducing and ammonifying properties and the ability to form hydrogen sulfide. According to the Bergey's Manual of Systematic Bacteriology the strain was identified as Bacillus sp. on the basis of the complex of microbiological, physiological and biochemical properties. 16S rRNA gene nucleotide sequence of the ChNPU ZVB1 strain was deposited in the GenBank database as Fictibacillus sp. KX349222. To define the phylogenetic position the dendrogram of relationship between various Bacillus and Fictibacillus species was constructed. Based on the obtained results the ChNPU ZVB1 strain belongs to the Fictibacillus genus and its species identification require additional molecular-genetic analysis.